Open Access

SAMPLE PREPARATION

General Information

These instruments are setup for open access: 

• Agilent 1100 HPLC with fraction collector (here or new page?) 
• Agilent 6120 Quadruple LC/MS with ApCI/ESI and fraction collection (coming soon)
• Agilent 6210 Time-of-Flight LC/MS
• Agilent 6890 GC/MS
• Applied Biosystems Vision Preparative Scale HPLC (here or new page?)
• Applied Biosystems Voyager DE-Pro MALDI-TOF
• Waters Quattro micro GC/MS/MS

Open Access (OA) provides a "Do It Yourself" mechanism for users to acquire mass spectra. Sample information is entered into a "Login Interface" by the user. Samples are prepared in auto injector vials and placed in the instrument's auto injector rack as instructed by the login system. Data are printed out after each sample has been analyzed. You can also log in a batch of samples.  Samples need to be compatible with the instrumental solvent system and ionization technique to achieve the best results.

Submission Sheet

No submission sheet required.  All user information is pre-entered and login is created.

Submission Process

Samples are prepared in solution in an auto-sampler vial.  Vials must be compatible with Agilent HPLC or GC auto-samplers. Samples are dissolved in an appropriate solvent (see FAQ for compatibility issues). Obtaining a reasonable sample concentration is crucial.  No more than a few ppm (parts per millions) should enter the instrument. This is because too much sample will cause a number of problems.

• You may clog the inlet system.
• You may contaminate the ion source of the instrument. This in turn may lead to carryover of ions from one sample to the next, so that ions from your sample appear in the spectrum of the following sample. Severe contamination may require physical cleaning of the ionization source which may lead to longer turn-around time.
• To discourage overloading, a service charge will be applied to your account if the instrument needs to be cleaned from your sample.  Concentration is sample dependent, but as a rule of thumb, please consults the following chart for starting concentrations.